Abstract
MicroRNAs are known to play essential role in the gene expression regulation in cancer. In our research, next-generation sequencing technology was applied to explore the abnormal miRNA expression of oral squamous cell carcinoma (OSCC) in Chinese hamster. A total of 3 novel miRNAs (Novel-117, Novel-118, and Novel-135) and 11 known miRNAs (crg-miR-130b-3p, crg-miR-142-5p, crg-miR-21-3p, crg-miR-21-5p, crg-miR-542-3p, crg-miR-486-3p, crg-miR-499-5p, crg-miR-504, crg-miR-34c-5p, crg-miR-34b-5p and crg-miR-34c-3p) were identified. We conducted functional analysis, finding that 340 biological processes, 47 cell components, 46 molecular functions were associated with OSCC. Meanwhile the gene expression of Caspase-9, Caspase-3, Bax, and Bcl-2 were determined by qRT-PCR and the protein expression of PTEN and p-AKT by immunohistochemistry. Our research proposed further insights to the profiles of these miRNAs and provided a basis for investigating the regulatory mechanisms involved in oral cancer research.
Highlights
As a part of head and neck squamous cell carcinoma (HNSCC), oral cancer, which is a class of common cancer in the world today, usually arises in the floor of the mouth, anterior two-thirds of the tongue, upper and lower alveolar ridges, lips and buccal mucosa, and so on[1,2]
Clinical treatment and prognosis are mainly based on the pathological classification of tumors, it does not provide the information about therapeutic biology and related molecular mechanisms[14]
Chinese hamster (Cricetulus griseus), has a special cheek pouch tissue which is similar to human oral mucosa, so it has distinctive advantage in the research of oral diseases
Summary
Differential expression analysis of known and novel miRNAs in the cancer and normal samples. We performed qRT-PCR analysis on the 5 significantly differentially expressed known miRNAs and 3 significantly differentially expressed novel miRNAs. Compared with the normal group, crg-miR-130-3p (7.372 ± 0.3416 vs 1.085 ± 0.06151), crg-miR-142-5p (5.228 ± 0.07653 vs 1.117 ± 0.01856), crg-miR-21-3p (14.250 ± 0.5306 vs 1.214 ± 0.08204), and crg-miR-34c-3p (5.598 ± 0.2093 vs 1.043 ± 0.04295) increased extremely significant (p < 0.001) and had relatively high and stable expression levels in the cancer group.
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