IDENTIFICATION AND PHARMACOLOGICAL PROFILING OF PLANT PEPTIDES AS INHIBITORS OF CLOSTRIDIUM BOTULINUM NEUROTOXINS A AND B

Abstract

Objective: Clostridium botulinum causes botulism which is a neuroparalytic disease caused by neurotoxins that are caused all over the world. This disease is perceived as a food born disease caused by a potent neurotoxin. Plant peptides are those referred to as proteins at a length of small than 100 amino acids. Plants have antimicrobial properties present in almost all plant species. These plant peptides are classified based on their primary structures. Methods: Molecular docking was implemented to evaluate the efficacy of nine phytocompounds from C. botulinum against the target proteins (3nf3, 2npo). PyRx, a Virtual Screening software was utilized for molecular docking which allowed the inspection of three-dimensional protein structures and the identification of potential binding sites. Result: On analyzing the molecular docking result of puroindoline, impatiens exhibited the best binding affinity toward the two target proteins. Conclusion: The safe and efficient treatment for some facial pleats and lines is botulinum toxin. High levels of patient and physician satisfaction are linked to its use. This paper has extensively revised three aspects the binding activity, models, and their phytochemical properties. The most significant results involve solvent interactions, van der Waals interactions, electrostatic interactions, and hydrogen bonding were analyzed and concluded in this research paper.