Abstract
Infectious bursal disease virus (IBDV) is a non-enveloped, bi-segmented double-stranded RNA virus and the causative agent of a poultry immunosuppressive disease known as infectious bursal disease (IBD). The novel variant IBDV (nVarIBDV) recently posed a great threat to the development of the poultry industry. In this study, we identified a novel segment-reassortant IBDV strain, IBDV-JS19-14701 (Genotype A2dB3). Phylogenic analysis showed that Segments A and B of IBDV-JS19-14701 were derived from emerging nVarIBDV (Genotype A2dB1) and long-prevalent HLJ0504-like strains (Genotype A3B3) in China, respectively. The pathogenicity of IBDV-JS19-14701 was further evaluated via animal experiments. IBDV-JS19-14701 exhibited a similar virulence to chickens with the nVarIBDV. The identification of this reassortment event is beneficial for understanding the epidemiology of nVarIBDV and will contribute to the efficient prevention and control of IBD.
Highlights
Infectious bursal disease (IBD) is one of the most important immunosuppressive poultry infectious diseases in poultry and has caused enormous economic losses to the poultry industry worldwide
RT-PCR targeting the hypervariable region (HVR) of VP2 confirmed that all five bursa samples were positive for infectious bursal disease virus (IBDV)
The final consensus genomic sequence of IBDV-JS19-14701 was determined by Sanger sequencing
Summary
Infectious bursal disease (IBD) is one of the most important immunosuppressive poultry infectious diseases in poultry and has caused enormous economic losses to the poultry industry worldwide. The etiological agent of IBD is infectious bursal disease virus (IBDV), which belongs to the genus Avibirnavirus in the family Birnaviridae [1]. The genome of IBDV is composed of two segments of double strand RNA, known as Segments A and B. Segment A encodes a VP5 and a polyprotein (pVP2-VP4-VP3). This polyprotein is self-cleaved by VP4 to produce VP2, VP3, and VP4 [2,3]. Segment B encodes VP1, an RNA-dependent RNA polymerase (RdRp) that is responsible for genome replication, translation, and viral virulence of IBDV [5]
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