Identification and partial purification of a chromatin bound calmodulin activated histone 3 kinase from calf thymus

Abstract

A calcium-calmodulin (Ca 2+-CaM) stimulated histone H3 phosphorylating activity was identified as a component of a nuclear protein complex purified from a 150 mM NaCl extract of calf thymus chromatin. This activity bound to a CaM-Sepharose affinity column in a Ca 2+ dependent manner and was eluted off the column in the presence of EGTA. Equilibrium centrifugation of the EGTA eluate on a sucrose density gradient revealed that the activity is a component of a larger complex identified at 25% sucrose. This complex consisted of two major proteins, having M r of 65 and 75 kDa. Using [ 125I] CaM and the gel overlay technique it was shown that the 75 kDa protein is the major CaM binding protein in the complex.