Identification and molecular characterization of Chryseobacterium vrystaatense ST1 isolated from oligomineral water of southeast Serbia

M Kojic,D Obradovic,S Tasic,S Stankovic

doi:10.2298/abs1203877t

Abstract

The isolation and molecular characterization of bacterial strains isolated from water sources in the Vlasina Mountain in southeast Serbia, confirmed the presence of a new species Chryseobacterium vrystaatense ST1. This Gram- negative species showed an extremely low level of biochemical reactivity in biochemical tests. The gene for 16S rRNA was amplified by PCR using universal primers and sequenced. Comparison of 16S rRNA gene sequence and phenotypic features indicated that the isolate ST belonged to Chryseobacterium vrystaatense. A BLAST search of sequenced 1088 nucleotides of the 16S rRNA gene with all sequences deposited in the NCBI collection showed the highest similarity (98%) with the strain Chryseobacterium vrystaatense sp. nov., designated as strain R-23533. The very high homology of these two strains allowed classification of our strain at the species level, but some differences indicate, and indirectly confirm, that the isolate ST is an authentic representative. On the basis of these results, we could conclude that Chryseobacterium vrystaatense ST was for first time isolated in Serbia, which is particularly important when one bears in mind that there are only three sequences of this species deposited in the NCBI collection.

Highlights

Physical and chemical characteristics water The spring water from which Chryseobacterium vrystaatense ST1 was isolated is characterized by its uniform flow (0.3 l/s), low mineralization (< 50 mg/l), pH=6.4 and low temperature (5.80C)
ST1 from water Using a membrane filtration technique for concentrating the bacteria in the water and growth of filter on nutrient agar for 48 h at 300C, ten different types of bacteria were isolated according to different characteristics of colonies
Chryseobacterium vrystaatense sp. nov. is a bacteria that acquired the status of new species in September 2005

Summary

Introduction

Various groups of microorganisms are found in drinking water as part of its indigenous microflora, but many of them, because of a lack of adequate methods, are not isolated or properly identified (Ishida et al, 1982). The standard identification methods used for the isolation and identification of bacteria are limited (Gerhardt et al, 1981). Most researchers use cultivation-independent molecular techniques for the monitoring of bacteria in the environment. Molecular methods are used together with cultivation methods in order to prove or to assist in species determination

Methods

Results

Conclusion