Identification and metabolic characterization of the Zea mays mitochondrial homolog of the Escherichia coli groEL protein

Abstract

We have characterized an abundant mitochondrial protein from Zea mays and have shown it to be structurally and metabolically indistinguishable from a previously described Tetrahymena thermophila and Saccharomyces cerevisiae mitochondrial protein, referred to as hsp60, which is homologous to the groEL protein of Escherichia coli. This Z. mays protein, which we also refer to as hsp60, was found to be antigenically quite distinct from the chloroplast Rubisco-binding protein, another groEL homolog. Using an antiserum directed against the T. thermophila hsp60, we determined that the relative concentration of Z. mays hsp60 was two to four times higher in mitochondria isolated from tissues of early developmental stages than that found in mitochondria isolated from more adult tissues. Given the known and suggested roles of the other members of the groEL family of proteins, our results suggest that the Z. mays hsp60 may play an important role in mitochondrial biogenesis during early plant development.