Abstract
Liriomyza trifolii (Diptera: Agromyzidae) is a leafminer that causes ruinous damage to many leafy vegetables including lettuce (Lactuca sativa L.) by stippling and tunneling the leaves. In this study, a population of 125 F3 families was developed from the intraspecific cross of ‘Valmaine’ (resistant) and ‘Okeechobee’ (susceptible) romaine cultivars for inheritance analysis and molecular mapping of the resistance loci controlling stippling damage. The experiments were conducted in an insectarium (controlled environment). Stippling damage proved to be heritable because the broad-sense heritability (H2) was 0.58. A segregation analysis suggested that a single dominant allele, Sd1 locus, controls resistance against L. trifolii. Furthermore, a quantitative trait loci (QTL) analysis identified one novel QTL, named Stippling on LG5 (qSTP5), flanked by two SNPs that were mapped to a 5.2 cM (8.5 Mb region) interval, explaining over 13% of the total phenotypic variance. Desirable allele for resistance to L. trifolii was derived from resistant cultivar Valmaine. Identification of SNPs closely linked to the QTL responsible for L. trifolii resistance should facilitate plant breeders to develop resistant romaine lettuce cultivars.
Highlights
Lettuce is one of the most important vegetable crops grown in the United States in terms of production and consumption[1]
Resistance sources have been incorporated into elite lettuce cultivars for California production[23], but not for Florida production because of the existence of efficacious insecticides to control this pest in Florida, and diseases caused by many microorganisms are more detrimental to lettuce production
This is the first attempt to determine the inheritance of the resistance against a devastating pest for lettuce, the serpentine leafminer, Liriomyza trifolii
Summary
Lettuce is one of the most important vegetable crops grown in the United States in terms of production and consumption[1]. In the U.S, lettuce is frequently attacked by several insect pests, one of which is L. trifolii[2,3,4] Besides lettuce, this polyphagous s pecies[5] attacks as many as 25 plant families with preference on the A steraceae[6] where lettuce belongs. This polyphagous s pecies[5] attacks as many as 25 plant families with preference on the A steraceae[6] where lettuce belongs This pest causes major economic losses in both ornamental and vegetables crops such as Chrysanthemums[7] and c elery[8], and o nion[9]. The objectives of this study were to (i) determine the mode of L. trifolii resistance in romaine lettuce and (ii) map the underlying resistance loci
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