Abstract
The use of sterility is common in plants and multiple loci for hybrid sterility have been identified in crops such as rice. In soybean, fine-mapping and research on the molecular mechanism of male sterility is limited. Here, we identified a male-sterile soybean line, which produces larger, abnormal pollen grains that stain poorly with I2-KI. In an inheritance test, all F1 plants were fertile and the F2 and F2:3 populations conformed with the expected segregation ratio of 3:1 (fertility:sterility) (p = 0.82) and showed a 1:2:0 ratio of homozygous fertile: heterozygous fertile: homozygous sterile genotypes (p = 0.73), suggesting that the sterility was controlled by a single recessive gene (designated “mst-M”). Bulked segregant analysis showed that almost all single-nucleotide polymorphisms (SNPs; 95.92%) were distributed on chromosome 13 and 868 SNPs (95.81%) were distributed in the physical region of Chromosome 13.21877872 to Chromosome 13.22862641. Genetic mapping revealed that mst-M was flanked by W1 and dCAPS-1 with genetic distances of 0.6 and 1.8 cM, respectively. The order of the consensus markers and known sterility genes was: Satt146 – (5.0 cM) – st5 – (2.5 cM) – Satt030 – (15.3 cM) – ms6 – (5.0 cM) – Satt149 – (39.5 cM) – W1 – (0.6 cM) – mst-M – (14.1 cM) – Satt516 (7.5 cM) – ms1 – (16.3 cM) – Satt595. These results suggest that mst-M is a newly identified male-sterility gene, which represents an alternative genetic resource for developing a hybrid seed production system for soybean.
Highlights
Sterility is a common phenomenon among plants
In comparison to female sterility, male sterility, including cytoplasmic male sterility (CMS) and genetic male sterility (GMS), has wide applications in commercial crop hybrids because male sterility greatly increases the effectiveness of F1 hybrid seed production without manual pollination and can dramatically reduce production costs (Cheng et al, 2007; Xu et al, 2007; Chen et al, 2011; Huang et al, 2014)
The results showed that the average total number of pollen grains per fertile wild-type parent (F-wt) flower was about 580, whereas St-M flowers (150) had about one-quarter the pollen grains of F-wt (Figure 2A)
Summary
Sterility is a common phenomenon among plants. On the basis of the mode of inheritance, two main types of sterility have been identified in plants: cytoplasmic sterility and nucleus-dependent sterility (Zhang et al, 2008; Chen and Liu, 2014; Yang et al, 2014; Speth et al, 2015; Bohra et al, 2016; Chang et al, 2016; Liu et al, 2018; Xie et al, 2018). In comparison to female sterility, male sterility, including cytoplasmic male sterility (CMS) and genetic male sterility (GMS), has wide applications in commercial crop hybrids because male sterility greatly increases the effectiveness of F1 hybrid seed production without manual pollination and can dramatically reduce production costs (Cheng et al, 2007; Xu et al, 2007; Chen et al, 2011; Huang et al, 2014). The most successful application of male sterility in crop hybrid seed production is in rice (Oryza sativa; Cheng et al, 2007; Huang et al, 2014). Many male sterility genes have been identified and cloned, and the underlying
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