Abstract
Rice line IR65482 has broad-spectrum resistance to Magnaporthe oryzae from different areas, and it has been known that there is a blast resistance gene Pi40 ( t ) on its chromosome 6. In the present study, using a bulked segregant analysis sequencing strategy, we identified another rice blast resistance gene on chromosome 11 of IR65482. The F 2 population derived from a cross between IR65482 and Nipponbare was further used for gene mapping, and the IR65482 blast resistance gene was mapped in a 425 kb region between InDel markers OSL3-2 and OSL3-5 at the end of chromosome 11. The results could provide guidance for application of IR65482 in breeding rice varieties with blast resistance and theoretical reference for the cloning of IR65482 blast resistance gene in the future.
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