Abstract
A method for tissue localization of hyaluronidase by a substrate film was used to study the time course and location of enzymatic activity in healing open rabbit wounds. Optically clear areas which were devoid of toluidine blue metachromasia indicated degradation of hyaluronate by hyaluronidase. Central granulation tissue exhibited hyaluronidase activity on the seventh postwound day, while whole edge tissue did not show activity until Day 18. The current work also compares the healing wound to other developmental systems in which hyaluronic acid has been implicated in morphogenesis, and where the appearance of hyaluronidase has been correlated to cytodifferentiation.
Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
