Identification and Isolation of the Bacterial Content in Stored Pap (Ogi/Akamu) in Masaka Market Nasarawa State of Nigeria

Abstract

The sole aim of this study was to determine, identify and isolate the bacterial content in stored Pap (Ogi/Akamu).This study was conducted in Masaka a conurbation of towns under Karu Local Government Area of Nasarawa State. Sterilization of glass wares with a disinfectant before and after use. Sample collection, media preparation, inoculation, incubation, isolation of pure culture were all aseptically carried out: For further identification, each typical colony was further sub cultured on MacConkey agar, peptone water agar, triple sugar iron agar, relevant biochemicals and Gram staining done for final confirmation . Analysis of the stored pap (ogi) from the four sellers at Masaka market as cultured on the nutrient agar and MacConkey agar showed the different bacteria and their percentages of occurrence as follows; Escherichia coli (12%), Staphylococcus aureus (34%), Pseudomonas spp(12%), Klebsiella spp(8%), Shigella(6%), Providensia nisera(8%), Staphylococcus capitis(14%) and Salmonella spp(6%). These distributions were not significant at 95% confidence limits. (P>0.05). In conclusion the present study showed that the quality of pap sold at Masaka community was poor. This was proven by the high bacteria count. It is suggested that means of collection and preparation of pap and the effect of unsterile containers for storage to be critically examined and improved upon.