Identification and isolation of flavonoids from Iraqi Silybum marianum L.flowers by HPLC

Abstract

One of the most important phytochemical compounds with potential applications in medicinal chemistry is the flavonoids which exist in many different parts of plants(fruits, herbs, vegetables). Flavonoids possess many medicinal benefits, including antioxidant, antiviral, anticancer, and anti-inflammatory properties. In addition to the cardio and neuro-protective effects. So the present study aimed to identify and isolate flavonoids from the flowers of Iraqi Silybum marianum L. by High-Performance Liquid Chromatography-HPLC. The results indicate that the HPLC analysis of plant flavonoids indicate that the flowers contain different concentration of flavonoids which include 119.7143 µg/g of Taxifolin, 307.4991 µg/g of Silychristin A, 137.6423 µg/g of Silidianin, 252.938 µg/g of Silychristin B, 339.9172 µg/g of Siliybin A, 378.3294 µg/g of Silybin B, 234.6421 µg/g of Isosilybin A and 127.2572 µg/g of Isosilybin B. The results also indicate that the identification of flavonoids in isolated part are the same in the crude flowers but different concentration, 138.0556µg/g of Taxifolin, 283.0717µg/g of Silychristin A, 122.4808µg/g of Silidianin, 190.1345µg/g of Silychristin B, 310.9728µg/g of Siliybin A, 363.0253µg/g of Silybin B, 214.0452µg/g of Isosilybin A and 126.3279µg/g of Isosilybin B.