Identification and growth-promoting effect of endophytic bacteria in potato

Abstract

In agriculture, Bacillusspecies are efficient and ecologically tool for promote the growth of theplant.Purpose: This study obtains the plant growth-promoting (PGP) ability ofendophytic bacteria isolated from the potato tubers.Methods: Using endophyticbacteria to promote potato growth, achieve the purpose of increasing production.In this experiment, the growth- promoting ability of the strain was verified bylaboratory identification and field test validation.Result: The isolateswere identified as Bacillus species based on a 16S rRNA gene sequenceand gyrB gene sequence analysis. DNA hybridization finally identified it as Bacillusvelezensis. Among the PGP attributes, the strain K-9 was found to be positivefor indole acetic acid (IAA) production, phosphate solubilization, siderophoreproduction, and nitrogen fixation. The isolate was found negative for potassiumsolubilization. The quantitative estimation of IAA product to 9.09 μg/ml. Theisolate also had the ability to produce lytic enzymes such as amylase andprotease. The quantitative estimation of protease activity is 89.16 μg/ml. The inoculation strain K-9 improved bioaccumulation of rootsand buds and yield in the potato compared to uninoculated control plants.Conclusion: These findings give an insight into the ways to use PGP bacteria to increasepotato production.