Abstract
Imperata cylindrica (L.) Raeusch. (IMP) aerial part ethyl acetate extract has anti-proliferative, pro-apoptotic, and pro-oxidative effects towards colorectal cancer in vitro. The chemical constituents of IMP aerial part ethyl acetate extract were isolated using high-performance liquid chromatography (HPLC) and identified with tandem mass spectrometry (ESI-MS/MS) in combination with ultraviolet-visible spectrophotometry and 400 MHz NMR. The growth inhibitory effects of each identified component on BT-549 (breast) and HT-29 (colon) cancer cell lines were evaluated after 48/72 h treatment by MTT assay. Four isolated compounds were identified as trans-p-Coumaric acid (1); 2-Methoxyestrone (2); 11, 16-Dihydroxypregn-4-ene-3, 20-dione (3); and Tricin (4). Compounds (2), (3), and (4) exhibited considerable growth inhibitory activities against BT-549 and HT-29 cancer cell lines. Compounds (2), (3), and (4) are potential candidates for novel anti-cancer agents against breast and colorectal cancers.
Highlights
Imperata cylindrica (L.) Raeusch. (IMP) is widely used for the treatment of hemorrhage, improvement of urination, and enhancement of the immune system [1]
The present study aims to isolate the chemical constituents from IMP aerial part ethyl acetate extract and identify the bio-active compounds with considerable growth inhibitory activity against cancers
Chemical structures of four compounds isolated from IMP aerial part ethyl acetate extract are Isolation, and Quantification of Compounds (1)–(4)
Summary
Imperata cylindrica (L.) Raeusch. (IMP) is widely used for the treatment of hemorrhage, improvement of urination, and enhancement of the immune system [1]. (IMP) is widely used for the treatment of hemorrhage, improvement of urination, and enhancement of the immune system [1]. Amounts of bio-active compounds isolated from IMP rhizomes and leaves were identified including benzoic acid and its derivatives [2], lignans [3], phenolic compounds [4], steroids [5], methoxylated flavonoids [6], and chromones [7]. Cancer is one of the leading causes of death worldwide. Herbal medicines are commonly used as both complementary ingredients and alternative therapies in cancer treatments. Potential bio-active components from herbal medicines can be isolated and purified using a high-performance liquid chromatography (HPLC) system. A tandem MS/MS detection system providing fragmentation information of the targets is one of the best choices adopted in chemical structural characterization and drug discovery [8,9]
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