Identification and genetic inheritance of a new source of broad-spectrum extreme resistance to turnip mosaic virus (TuMV) in Brassica rapa

Abstract

Brassica rapa line K185 was identified with broad-spectrum extreme resistance to turnip mosaic virus (TuMV). The resistance was effective against TuMV isolates UK 1, CZE 1, GBR 6, POL 1 and CDN 1 (representing major pathotypes 1, 3 and 4 and major resistance-breaking isolates) following mechanical inoculation. F1 plants from a cross between K185 and the rapid-cycling B. rapa ssp. trilocularis line R-o-18 (uniformly susceptible to all the above TuMV isolates), were resistant following challenge with TuMV isolates CDN 1 and GBR 6, indicating the involvement of dominant gene(s). F2 plants derived from a single F1, CDN 1- and GBR 6-resistant plant segregated for resistance to TuMV isolate GBR 6. The segregation ratio of resistant to susceptible plants was consistent with at least two loci controlling resistance and with both loci having dominant alleles for resistance. Other sources of broad-spectrum resistance to TuMV have been shown to involve the recessive allele retr01. The broad-spectrum resistance in K185 clearly involves different alleles to retr01 and provides the opportunity to introgress an alternative form of TuMV resistance into commercial B. rapa lines and thereby reduce the selection pressure for retr01 resistance breaking.