Identification and gene mapping of a narrow and upper-albino leaf mutant in rice (Oryza sativa L.)

Feng Wang,Guanghua He,Runlong Miao,Tingting Lin,Yanqiang Tang,Xianchun Sang,Fangfang Xu

doi:10.1007/s11434-012-5154-7

Feng Wang, Guanghua He + Show 5 more

Open Access

https://doi.org/10.1007/s11434-012-5154-7

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Journal: Science Bulletin	Publication Date: Apr 30, 2012
Citations: 7	License type: CC BY 2.0

Affiliation: Southwest University, Rice Research Institute

Abstract

The leaf blade consists of color and shape traits. Studies of leaf-blade development are important for improvement of rice yield and quality because it is an essential organ for photosynthesis. A narrow and upper-albino leaf mutant (nul1) was identified from among progeny of the indica restorer line Jinhui10 raised from seeds treated with ethyl methane sulfonate. Under field conditions, the mutant displayed narrow and upper-albino leaf blades with significantly decreased photosynthetic pigment contents throughout their development. The narrow-leaf trait is caused by a decreased number of small veins. In contrast to the wild type, the growth period was extended by approximately 8 d and agronomic traits, such as effective panicle number, percentage seed set and 1000-grain weight, declined significantly in the nul1 mutant. Genetic analysis suggested that the narrow and upper-albino leaf characteristics showed coseparation and were controlled by one recessive gene. The Nul1 gene was mapped onto chromosome 7 between the Indel marker Ind07-1 and the Simple Sequence Repeat marker RM21637. The physical distance between the markers was 75 kb and eight genes were annotated in this region based on the rice Nipponbare genome sequence. These results provide a foundation for cloning and function analysis of Nul1.

Highlights

Rice Research Institute, Chongqing Key Laboratory of Application and Safety Control of Genetically Modified Crops, Engineering Research Center of South Upland Agriculture, Ministry of Education, Southwest University, Chongqing 400716, China
*Corresponding author present, more than 90 leaf-color mutants have been identified in rice, and the responsible genes cloned from these mutants have been shown to function in, for example, chlorophyll synthesis and degradation, chloroplast growth, and the programmed cell death process [2–7]
Under the natural field conditions, the nul1 mutant exhibited an abnormal leaf color and leaf shape compared with the wild type (Figure 1(a) and (b))

Summary

Introduction

Rice Research Institute, Chongqing Key Laboratory of Application and Safety Control of Genetically Modified Crops, Engineering Research Center of South Upland Agriculture, Ministry of Education, Southwest University, Chongqing 400716, China. Studies of leaf-blade development are important for improvement of rice yield and quality because it is an essential organ for photosynthesis. The mutant displayed narrow and upper-albino leaf blades with significantly decreased photosynthetic pigment contents throughout their development. The physical distance between the markers was 75 kb and eight genes were annotated in this region based on the rice Nipponbare genome sequence These results provide a foundation for cloning and function analysis of Nul. The development of leaf color, size, and shape can directly or indirectly influence the proportion of sunlight energy utilized and have an important effect on yield and grain quality of cultivated rice. More than 90 leaf-color mutants have been identified in rice, and the responsible genes cloned from these mutants have been shown to function in, for example, chlorophyll synthesis and degradation, chloroplast growth, and the programmed cell death process [2–7]. Only seven narrow-leaf mutants are reported to be controlled by a single gene (nal, nal, nal, nal, nal, nal and nal7), of which Nal and Nal

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