Abstract
The buffalo population is extensive in China, but its meat quality is relatively inferior. Therefore, improving meat quality should be one of the breeding goals. microRNAs (miRNAs) play an essential regulatory role in the post-transcriptional expression of genes. Some studies have reported their function regulating genes related to fat deposition and adipocyte differentiation in cattle, but there is limited reports in buffalo. We performed small RNA transcriptome sequencing of Xinyang buffalo adipose tissue between calves and adults in this study. As a result, 282 mature miRNAs were significantly differentially expressed, and co-expression analysis showed that 454 miRNAs were significantly associated with developmental stages. Target gene identification, GO (gene ontology) annotation, and KEGG analysis of miRNAs showed that miR-195, miR-192, and miR-24-3p could target key genes for lipogenesis and thus regulate adipose deposition and differentiation. Among them, miR-195 was significantly upregulated in adipose tissue and induced adipocytes of adult buffaloes, and its overexpression significantly inhibited lipid accumulation in primary adipocytes. Dual-luciferase reporter gene analysis showed that miR-195 reduced the expression of thyroid hormone response protein (THRSP) by targeting its 3′ untranslated terminal region, suggesting that miR-195 may inhibit lipid accumulation in adipocytes by regulating THRSP. The results confirmed the reliability of predictive screening of miRNAs and provided theoretical support for buffalo fattening.
Highlights
IntroductionSeveral studies have shown that miRNAs regulated (activation and inhibition) adipocyte differentiation and lipid accumulation (Remsburg et al, 2019)
Fat formation is a highly complex process in mammals
Domestic buffalo are mainly distributed in Asian countries and are classified into Bubalus bubalis and Bubalus carabanensis based on morphology, behavior, and karyotype
Summary
Several studies have shown that miRNAs regulated (activation and inhibition) adipocyte differentiation and lipid accumulation (Remsburg et al, 2019). Over-expression of miR-33b could reduce proliferation, impair differentiation, and decrease lipid droplets in precursor adipocytes (Price et al, 2016). Promoting the meat performance of Xinyang buffalo is a critical way to improve its breeding status and increase its quantity. Given the comprehensive and vital roles of miRNAs in fat deposition and lipid accumulation, their expression profiles in humans, mice, pigs, cattle, and buffalo (Sun et al, 2014; Guller et al, 2015; Oclon et al, 2016) have recently been characterized in adipose tissue or adipose-associated cells, providing essential information for further research on fat deposition. Details of the expression profiles and mechanisms of miRNAs in fat accumulation in buffalo are limited and require further investigation
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