Abstract
利用cDNA-AFLP技术筛选到1个在抗黄矮病的小麦-中间偃麦草易位系YW642中特异表达的小麦抗病基因类似序列(BYDV resistance-related gene1, BRG1 )的基因片段。利用cDNA末端快速扩增技术(RACE)和RT-PCR技术，从YW642中分离出 BRG1 基因全长cDNA序列，获得了一个通读的抗病同源基因cDNA序列，编码由645个氨基酸组成的蛋白质，包含1个NB-ARC保守结构域和3个LRR结构域，该蛋白属于nucleotide-site binding, leucine-rich repeats (NBS-LRR)家族。荧光定量或半定量RT-PCR表达分析表明， BRG1 在抗病小麦易位系YW642叶片中优势表达，受BYDV的诱导，BYDV接种后48 h表达量最高， BRG1 在感病小麦亲本中8601中表达量始终较低，随BYDV接种时间延长呈轻微的下调趋势；而且外源激素水杨酸(SA)与茉莉酸(JA)处理可上调该基因在YW642中的表达。利用病毒介导的基因沉默技术分析了 BRG1 基因的功能，结果表明该基因沉默的抗病小麦易位系YW642中BYDV相对含量增加，但未造成抗病性表型显著改变，说明该基因参与抗黄矮病反应，但不是小麦抗黄矮病重要基因。
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