Identification and fine mapping of qPBR10-1, a novel locus controlling panicle blast resistance in Pigm-containing P/TGMS line.

Yunyu Wu,Ling Yu,Wei Shi,Qiang Gao,Yuese Ning,Hongjuan Ji,Chengzhi Liang,Jianju Liu,Changhai Zhou,Xiaoxiang Zhang,Yue Cai,Cunhong Pan,Ning Xiao,Aihong Li,Yuhong Li,Niansheng Huang,Zichun Chen

doi:10.1007/s11032-021-01268-3

Abstract

The online version contains supplementary material available at 10.1007/s11032-021-01268-3.

Highlights

Rice blast is one of the most widespread and devastating diseases of rice and causes yield losses between 10 and 30% of rice production worldwide in epidemic years (Skamnioti and Gurr 2009)
MSJ13 and MSJ18 were obtained by successive backcrossing and inbreeding with introducing the broadspectrum resistance gene Pigm into C134S, an elite P/TGMS line widely used in two-line hybrid rice, through molecular marker assisted selection in 2013
We found that Pigm displayed constitutive expression in all tissues and showed equivalent expression patterns between MSJ13 and MSJ18 (Fig. 1b)

Summary

Introduction

Rice blast is one of the most widespread and devastating diseases of rice and causes yield losses between 10 and 30% of rice production worldwide in epidemic years (Skamnioti and Gurr 2009). Among the R genes identified and cloned, many broad-spectrum R genes have been documented and validated, viz., Pi1, Pi2, Pi5, Pi9, Pi33, Pi40, Piz, Piz-t, Pigm, and others (Jeung et al 2007; Wu et al 2007), and six of which (Pi2, Pigm, Pi40, Pi9, Piz and Piz-t) were different R gene alleles of the Piz locus located on the short arm near the centromere of rice chromosome 6 (Deng et al 2006; Qu et al 2006; Zhou et al 2006). The broad-spectrum resistance gene Pigm identified from landrace Gumei 4 (GM4), has been shown to be completely resistant to 50 isolates originating from diverse Chinese and worldwide collections, and was used as an excellent resistance resource in blast resistance breeding for more than 30 years in different varieties cultivated on large surfaces The broad-spectrum resistance gene Pigm identified from landrace Gumei 4 (GM4), has been shown to be completely resistant to 50 isolates originating from diverse Chinese and worldwide collections, and was used as an excellent resistance resource in blast resistance breeding for more than 30 years in different varieties cultivated on large surfaces (Deng et al 2017; Cesari and Kroj. 2017). Deng et al (2017) revealed that epigenetic regulation of Pi-gmR and Pi-gmS balance the blast resistance and yield in rice, in which Pi-gmR confer broad-spectrum resistance of GM4, and Pi-gmS increase rice production to counteract the yield lost caused by Pi-gmR

Methods

Results

Conclusion