Identification and Expression Profiling of Odorant Receptor Protein Genes in Sitophilus zeamais (Coleoptera: Curculionoidea) Using RT-qPCR.

Abstract

This study aimed to identify ORs (odorant receptors) and Orco (odorant receptor coreceptor) genes in Sitophilus zeamais Motschulsky (Coleoptera: Curculionoidea), to explore the relative expression levels of these genes in different adult tissues and obtain information on highly expressed receptor proteins. Putative OR and Orco genes were identified from transcriptomic data previously obtained for S. zeamais using bioinformatics methods. Quantitative real-time PCR was used to compare the differences in expression in seven adult tissues (male antennae, female antennae, heads, thoraxes, abdomens, wings, and legs). The candidate OR and Orco gene sequences were analyzed, and the protein physicochemical properties were predicted. We identified 64 OR genes including the Orco gene. Forty-seven OR genes, including Orco, were over expressed in male or female antennae. Seventeen OR genes appeared to be expressed at elevated levels in male antennae. Twenty-nine genes were expressed at significantly elevated levels in female antennae. In total, 11 OR genes were selected for further sequence analysis. The selected proteins were structurally characterized, and bioinformatics analysis was performed. Overall, in this study, candidate ORs of S. zeamais have been identified for the first time, and these ORs could be molecular targets for interference in the insect olfactory system.