Abstract
A cold-related protein, cold shock protein 3 (BcCSP3), was isolated from non-heading Chinese cabbage in this study. BcCSP3 can encode 205 amino acids (aa) with an open reading frame (ORF) of 618 base pairs (bp). Multiple sequence alignment and phylogenetic tree analyses showed that BcCSP3 contains an N-terminal cold shock domain and is highly similar to AtCSP2, their kinship is recent. Real-time quantitative polymerase chain reaction (RT-qPCR) showed that the expression level of BcCSP3 in stems and leaves is higher than that in roots. Compared with other stress treatments, the change in BcCSP3 expression level was most pronounced under cold stress. In addition, a BcCSP3–GFP fusion protein was localized to the nucleus and cytoplasm. These results indicated that BcCSP3 may play an important role in response to cold stress in non-heading Chinese cabbage. This work may provide a reference for the identification and expression analysis of other CSP genes in non-heading Chinese cabbage.
Highlights
Non-heading Chinese cabbage (Brassica rapa ssp. chinensis), a widely grown vegetable crop, is an important Brassica crop with exceptional cold resistance [1,2]
Through sequence alignment and evolutionary tree analysis, we found that BcCSP3 and AtCSP2 proteins are highly similar and have a closer relationship
Through Real-time quantitative polymerase chain reaction (RT-qPCR) analysis, we found that the expression level of BcCSP3 in stems and leaves was much higher than that in roots (Figure 6)
Summary
Non-heading Chinese cabbage (Brassica rapa ssp. chinensis), a widely grown vegetable crop, is an important Brassica crop with exceptional cold resistance [1,2]. Chinensis), a widely grown vegetable crop, is an important Brassica crop with exceptional cold resistance [1,2]. As one of the important environmental factors (abiotic stress), cold stress can affect crop yield and quality by restricting plant growth and development [4]. After a period of low temperature (no freezing), some plants can obtain higher resistance to freezing. This process is called cold acclimation and has been proven by previous studies [5]. Cold acclimation is a complex process with a series of changes in gene expression and protein metabolism [6]
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