Identification and Expression Analysis of Candidate Odorant-Binding Protein and Chemosensory Protein Genes by Antennal Transcriptome of Sitobion avenae.

Wenxin Xue,Julian Chen,Zongli Han,Yong Zhang,Jingrui Sun,Jia Fan,Qingxuan Xu

doi:10.1371/journal.pone.0161839

Wenxin Xue, Julian Chen + Show 5 more

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https://doi.org/10.1371/journal.pone.0161839

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Abstract

Odorant-binding proteins (OBPs) and chemosensory proteins (CSPs) of aphids are thought to be responsible for the initial molecular interactions during olfaction that mediate detection of chemical signals. Analysis of the diversity of proteins involved comprises critical basic research work that will facilitate the development of sustainable pest control strategies. To help us better understand differences in the olfactory system between winged and wingless grain aphids, we constructed an antennal transcriptome from winged and wingless Sitobion avenae (Fabricius), one of the most serious pests of cereal fields worldwide. Among the 133,331 unigenes in the antennal assembly, 13 OBP and 5 CSP putative transcripts were identified with 6 OBP and 3 CSP sequences representing new S. avenae annotations. We used qPCR to examine the expression profile of these genes sets across S. avenae development and in various tissues. We found 7 SaveOBPs and 1 SaveCSP were specifically or significantly elevated in antennae compared with other tissues, and that some transcripts (SaveOBP8, SaveCSP2 and SaveCSP5) were abundantly expressed in the legs of winged or wingless aphids. The expression levels of the SaveOBPs and SaveCSPs varied depending on the developmental stage. Possible physiological functions of these genes are discussed. Further molecular and functional studies of these olfactory related genes will explore their potential as novel targets for controlling S. avenae.

Highlights

The grain aphid, Sitobion avenae (Fabricius) is one of the most important pests of gramineous crops [1]
We report on the sequencing, assembly and partial annotation of anntenal transcriptomes for winged and wingless grain aphids (S. avenae) and we identified 13 Odorant-binding proteins (OBPs) and five chemosensory proteins (CSPs)
Nearly twice as many OBPs have been reported for A. lucorum (38 OBPs) and L. lineolaris (33 OBPs) [19, 25]

Summary

Introduction

The grain aphid, Sitobion avenae (Fabricius) is one of the most important pests of gramineous crops [1]. Damage from their sucking plant sap slows plant growth and reduces the number of tillers, severely diminishing the yield and quality of wheat [2]. Antennal Transcriptome of S. avenae and Odorant Binding Proteins and Chemosensory Proteins Analysis contributed to the writing of the manuscript, 3. The National Science and Technology Major Project (http://www.nmp.gov.cn/) 2014ZX0800201B. JF conceived and designed the experiments and contributed to the writing of the manuscript, and 5. Cooperation Project between China and Belgium from Ministry of Science and Technology (MOST) (http://www.istcp.org.cn/) 2014DFG32270. JLCH conceived and designed the experiments and decision to publish

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