Abstract
Homologous DNA probes with a variable degree of repetitivity were used to identify the precise relationships between isolates belonging to the pinewood nematodes. Southern blot hybridization patterns obtained with genomic DNA from 13 geographical isolates confirm the existence of a B. xylophilus group and a B. mucronatus group within these pinewood nematodes. With these probes we were also able to discriminate between geographical isolates. The results clearly indicate the existence of three geographical subgroups in the B. xylophilus species: the United States, Canada and Japan with a close relationship between the USA and Japanese isolates. This supports the hypothesis that the B. xylophilus isolate, which was introduced into Japan, originated from the USA. Furthermore, these probes clarify the taxonomic status of a Minnesota isolate found on Abies balsamea and of the French and Norwegian isolates of pinewood nematodes. The nature of the isolated sequences is also discussed.
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