Identification and distribution of tryptophan hydroxylase (TPH)-positive neurons in the planarian Dugesia japonica

Kaneyasu Nishimura,Yoshihisa Kitamura,Takeshi Inoue,Yoshihiko Umesono,Kanji Yoshimoto,Kosei Takeuchi,Takashi Taniguchi,Kiyokazu Agata

doi:10.1016/j.neures.2007.05.014

Identification and distribution of tryptophan hydroxylase (TPH)-positive neurons in the planarian Dugesia japonica

Kaneyasu Nishimura, Yoshihisa Kitamura + Show 6 more

https://doi.org/10.1016/j.neures.2007.05.014

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Journal: Neuroscience Research	Publication Date: Jun 3, 2007
Citations: 92

Affiliation: Kyoto Pharmaceutical University, KAN Research Institute, Kyoto University, Kyoto Prefectural University of Medicine

#Neurons In Planarians #Ventral Nerve Cords + Show 8 more

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Abstract

We identified a full-length tryptophan hydroxylase (TPH) gene of planarian Dugesia japonica from a head EST database, and named it DjTPH. Based on whole-mount in situ hybridization and immunofluorescence analyses, DjTPH mRNA and protein were mainly expressed in the nervous system, especially ventral nerve cords and eye pigment cells. Furthermore, DjTPH immunoreactivity was clearly detected at commissure axonal connections in the ventral nerve cords. 5-HT was significantly decreased in DjTPH-knockdown planarians compared with control animals. These results suggest that DjTPH is required for 5-HT biosynthesis, and DjTPH antibody is a useful marker for serotonergic neurons in planarians.

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