Abstract
Our laboratory demonstrated that endoplasmic reticulum iPLA 2 (ER-iPLA 2) activity protects renal cells from oxidant-induced cell death and lipid peroxidation. The goals of this study were to determine the PLA 2 isoform(s) responsible for ER-iPLA 2 activity in different species and tissues. ER-iPLA 2 activity was observed in microsomes from rabbit and rat kidney, heart, and brain as well as in human kidney (Caki-1 and HEK293) and glioblastoma (A172) cell lines. Reverse transcriptase-polymerase chain reaction results demonstrated the presence of iPLA 2γ (group VIB PLA 2) message in all tissues tested. Immunoblot analysis and PLA 2 inhibitor studies with methyl arachidonyl fluorophosphonate and enantiomers of bromoenol lactone demonstrated that the ER-iPLA 2 in rabbit kidney and heart and rat kidney is iPLA 2γ. These results demonstrate the expression of ER-iPLA 2γ (group VIB) across species and tissues, and suggest that iPLA 2γ may play critical roles in oxidant-induced cell injury.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callMore From: Biochemical and Biophysical Research Communications
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
