Identification and distribution of alleles of hybrid necrosis gene Ne2 in soft wheat cultivars (Triticum aestivum L.)

Abstract

Aim. To determine the correspondence of different alleles of locus Xbarc55-2В to alleles of gene Ne2, different in their strength, and to investigate the distribution of alleles of locus Xbarc55-2В and the corresponding alleles of gene Ne2 from wheat cultivars of Ukrainian and Russian selection. Methods. Polymerase chain reaction, electrophoresis in PAAG, statistical methods. Results. The alleles of locus Xbarc55-2В, bound to the hybrid necrosis gene Ne2, were used to identify the genotypes of 290 soft wheat cultivars of different geographic origin. The correspondence of different alleles of locus Xbarc55-2В to alleles of gene Ne2, different in their strength, was defi ned: 142 bp – ne2, 136 bp – Ne2 w/m , 132 bp – Ne2 ms , 126 bp – Ne2 s . The distribution of the identifi ed alleles of gene Ne2 among the wheat cultivars of Ukrainian and Russian selection was demonstrated. Conclusions. The advantage of some alleles for cultivars of different regions was determined, which may testify to their selection and adaptation value.