Identification and differentiation of Ricinus communis L. using SSR markers

Zdenka Gálová,Tí­Mea Kuťka Hlozáková,Želmí­Ra Balážová,Martin Vivodí­K

doi:10.5219/516

Zdenka Gálová, TíMea Kuťka Hlozáková + Show 2 more

Open Access

https://doi.org/10.5219/516

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Abstract

The castor-oil plant (Ricinus communis L.), a member of the spurge family (Euphorbiaceae), is a versatile industrial oil crop that is cultivated in many tropical and subtropical regions of the world. Castor oil is of continuing importance to the global specialty chemical industry because it is the only commercial source of a hydroxylated fatty acid. Castor also has tremendous future potential as an industrial oilseed crop because of its high seed oil content, unique fatty acid composition, potentially high oil yields and ability to be grown under drought and saline conditions. Knowledge of genetic variability is important for breeding programs to provide the basis for developing desirable genotypes. The aim of this study was to assess genetic diversity within the set of 60 ricin genotypes using 10 SSR primers. Ten SSR primers revealed a total of 67 alleles ranging from 4 to 9 alleles per locus with a mean value of 6.70 alleles per locus. The PIC values ranged from 0.719 to 0.860 with an average value of 0.813 and the DI value ranged from 0.745 to 0.862 with an average value of 0.821. Probability of identity (PI) was low ranged from 0.004 to 0.018 with an average of 0.008. A dendrogram was constructed from a genetic distance matrix based on profiles of the 10 SSR loci using the unweighted pair-group method with the arithmetic average (UPGMA). According to analysis, the collection of 60 diverse accessions of castor bean was clustered into six clusters. We could not distinguish 2 genotypes grouped in cluster 1, RM-96 and RM-98, which are genetically the closest. Knowledge on the genetic diversity of castor can be used to future breeding programs of castor.

Highlights

Castor (Ricinus communis L.) is a cross-pollinated diploid (2n = 2x = 20) species belonging to the family Euphorbiaceae and genus Ricinus
This study investigates the genetic diversity among 60 castor genotypes using 10 simple sequence repeat (SSR) markers
Results indicated the presence of wide genetic variability among different genotypes of castor

Summary

Introduction

Genetic variability in castor bean has been studied using molecular techniques, including amplified fragment length polymorphism (AFLP) (Pecina-Quintero et al, 2013), random amplified polymorphism DNA (RAPD) (Vivodík et al, 2014), single nucleotide polymorphism (SNP) markers (Foster et al, 2010), simple sequence repeat (SSR) (Tan et al, 2014), start codon targeted polymorphism (SCoT) and inter simple sequencerepeat (ISSR) (Kallamadi et al, 2015). Vivodík et al, (2014) used 8 RAPD markers to detect genetic variability among the set of 40 castor genotypes. Amplification of genomic DNA of 40 genotypes, using RAPD analysis, yielded in 66 fragments, with an average of 8.25 polymorphic fragments per primer. Among the DNA markers, SSR markers have been used intensively to analyse genetic diversity These markers are favourable as they exhibit high locus-specificity, high levels of variability, robustness towards genotyping, and a co-dominant mode of inheritance (Woodhead et al, 2005). Several investigations on the discrimination between crop genotypes using SSR markers have been carried out by Siripiyasing et al, (2013); Fayyaz et al, (2014); Kanwal et al, (2014); Polat et al, (2015); Yousaf et al, (2015)

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