Identification and Differentiation of Highly Diverse Campylobacter concisus Strains using the MALDI Biotyper

Hans Linde Nielsen,Mette Mølvadgaard

doi:10.4172/2327-5073.1000230

Abstract

In Danish diarrheic stool samples, the emerging enteric pathogen Campylobacter concisus has an incidence at the level of Campylobacter jejuni. Nevertheless, C. concisus has been neglected in many clinical laboratories due to labour intensive isolation procedures as well as time-consuming PCR-based methods for identification. Fourteen isolates of C. concisus were characterised in the MALDI-TOF mass spectrometer and analysed using the MALDI Biotyper and the ClinProTools 3.0 software. The data show that MALDI-TOF MS, as previously described for other Campylobacter species, can give a rapid identification of C. concisus. Interestingly, the isolates showed a remarkable diversity based upon their mass spectra when compared by visual and computational analysis.

Highlights

In Danish diarrheic stool samples, the emerging enteric pathogen Campylobacter concisus has an incidence at the level of Campylobacter jejuni
Campylobacter jejuni and other Campylobacter species can be identified to species level with use of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) [2]
Campylobacter concisus does not grow on modified charcoal-cefoperazone-deoxycholate agar which is routinely used for cultivation mCCDA in western countries

Summary

Introduction

In Danish diarrheic stool samples, the emerging enteric pathogen Campylobacter concisus has an incidence at the level of Campylobacter jejuni. Campylobacter concisus and other emerging Campylobacter species can be isolated in high numbers in stools by use of a filtration technique on 5% yeast-enriched blood agar plates (SSI Diagnostica, Hillerød, Denmark) incubated at 37°C in a microaerobic atmosphere with hydrogen [14]. Identification of C. concisus has until recently been based on PCR-based techniques which may seem slow compared to the rapid identification by use of the MALDI-TOF MS.

Results

Conclusion