Identification and differential expression of a sex-peptide receptor in Helicoverpa armigera

Abstract

Sex-pheromone production in the night flying female moth, Helicoverpa armigera is under neuroendocrine control due to the timely release of Pheromone Biosynthesis-Activating Neuropeptide (PBAN). Males orient to the females by upwind anemotaxis which usually leads to a successful mating. During copulation insect males transfer seminal peptides, produced in Male Accessory Glands (MAGs) which are implicated in post-mating behavioral changes of the females. These changes include the termination of pheromone biosynthesis and thus females do not re-mate. In previous studies we showed that synthetic Drosophila melanogaster Sex-Peptide ( DrmSP), which is responsible for terminating receptivity in female flies, can terminate PBAN-stimulated pheromone production by pheromone glands of the female moth, H. armigera. In addition, we demonstrated that at least one fraction of the H. armigera MAG extract is both immunoreactive to DrmSP antibody and is pheromonostatic, we also showed that different sets of DrmSP-like immunoreactive peptides are up-regulated in the central nervous system of mated females. In the present study, we identify a putative receptor for sex-peptide (SP-R) in H. armigera on the basis of sequence homologies deposited in the GenBank. In addition, in an attempt to draw some light on the physiological significance of SP-like peptides in this moth, we conducted a differential expression study of this receptor comparing gene expression levels in relation to different photoperiods, sex and mating status of the moth. Photoperiod and mating influence SP-R gene expression levels and sexual dimorphic changes were observed in neural tissues due to the different physiological states. After mating SP-R transcript levels in female neural tissues and pheromone glands are up-regulated. Physiological studies in vivo confirm the up-regulation of gene expression levels in pheromone glands isolated from mated females.