Abstract
A simple, sensitive, and specific high-performance liquid chromatography method has been developed for the simultaneous quantification of the three major biologically active ingredients, gastrodin (GA), 4-hydroxybenzyl alcohol (HA), and 4-hydroxybenzaldehyde (HD), in Gastrodia elata Blume for the first time. The HPLC assay was performed on a reversed phase ODS column by using methanol-water-isopropyl alcohol (35:55:10, v:v:v) as mobile phase with a flow rate of 0.4mL/min. The detection wavelength was set at 270 nm. Regression equations revealed linear relationships (correlation coefficients: 0.9997–0.9999) between the peak area of each constituent (GA, HA, HD) and its concentration. The relative standard deviations of the retention times of three constituents range between 0.2∼0.5%. The recoveries for the three constituents ranged between 96.6∼98.5%. The GA, HA, HD contents measured 12.0 mg/g (2.53% RSD), 2.1 mg/g (2.04% RSD), and 0.13 mg/g (2.63% RSD), respectively, in the ethanol extracts of Gastrodia elata Blume.
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