Abstract

An improved high-performance liquid chromatographic technique with photodiode-array detection was developed for the identification and determination of the active principles of Magnolia officinalis extracts such as honokiol and magnolol. A reversed-phase column (Nucleosil 7C 18) was eluted with a isocratic system of acetonitrile-0.1% phosphoric acid (63:35). It was found that 19.13 ± 0.62 mg of honokiol and 75.24 ± 3.48 mg of magnolol were contained in the methanol extracts of 1 g of Magnolia officinalis.

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