Abstract
Aim To investigate the absorbed components of Danggui-Shaoyao-San (DSS) in rat plasma and their pharmacokinetics. Methods Serum samples from rats with DSS extract administrated orally were analyzed by HPLC-DAD-MS n and an HPLC-DAD method was established for the determination. Results Seven prototypes and one metabolite were identified in rat serum, and albiflorin, paeoniflorin, ferulic acid and ligustilide in rat plasma were determined for pharmacokinetic study. The proposed method was validated with a linear range of 0.025–5.60 μg·mL −1 and limit of quantitation (LOQ) at 0.022–0.180 μg·mL −1, respectively. The RSD of intra- and inter-day precision variations were less than 10.1% and the accuracies ranged from 92.5%–111.5%. The overall recovery was over 80%. Conclusion Paeoniflorin sulfonate, albiflorin, paeoniflorin, ferulic acid, senkyunolide I, ligustilide and butylidenephthalide were found to be the potentially effective constituents in DSS. The method for determination is simple and sensitive, which is proved to be suitable for pharmacokinetic study of albiflorin, paeoniflorin, ferulic acid and ligustilide in DSS.
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