Abstract
To test RNA-RNA interactions in cells, we developed a yeast RNA hybrid system derived from the yeast three-hybrid system. In this setup, the activation of a reporter gene (HIS3 or lacZ) is dependent on the interaction of two RNAs. One ('RNA X') is fused to MS2 RNA, forming the bait, which binds to a fusion protein composed of the MS2 coat and the LexA proteins. The second ('RNA Y') is fused to an RNA-based transcriptional activator (m26-11), forming the prey. If prey (RNA Y) binds to bait (RNA X), the m26-11 transcriptional activator is tethered to the promoter of the reporter genes. This protocol describes how to use this RNA hybrid system. In addition to testing RNA-RNA interactions, it can also be used to screen RNA libraries to identify new interaction partners or for mutational analysis of two known interaction partners.
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