Identification and Detection of Botryosphaeria dothidea from Kiwifruit (Actinidia chinensis) in China.

Li Wang,Hui Hou,Hongbo Yuan,Hongtao Tu,Zengqiang Zhou

doi:10.3390/plants10020401

Abstract

Kiwifruit is very popular among consumers due to its high nutritional value. The increasing expansion in kiwifruit cultivation has led to the spread of rot diseases. To identify the pathogens causing kiwifruit ripe rots in China, 24 isolates were isolated from the diseased fruit and wart in trees. Botryosphaeria dothidea was recognized as the pathogen causing kiwifruit ripe rot and wart in the tree through internal transcribed spacer (ITS) sequencing, pathogenicity testing, morphological and microscopic characteristics. The rapid and accurate detection of this pathogen will lead to better disease monitoring and control efforts. A loop-mediated isothermal amplification (LAMP) method was then developed to rapidly and specifically identify B. dothidea. These results offer value to further research into kiwifruit ripe rot, such as disease prediction, pathogen rapid detection, and effective disease control.

Highlights

Identification and Detection of Kiwifruit (Actinidia chinensis) is a deciduous tree belonging to the Actinidiaceae family
Several fungi including Alternaria, Colletotrichum, Botryosphearia, Cylindrocarpon, Phomopsis, Phoma, Diaporthe, Botrytis, and Penicillium have been reported in association with kiwifruit rot [5,6,7,8,9,10,11]
The results of this study offer value to further research into kiwifruit ripe rot, such as disease prediction, pathogen rapid detection, and effective disease control

Summary

Introduction

Kiwifruit has been popular due to its balanced nutritional minerals, dietary fibers, high vitamin C contents, and health-promoting metabolites [1]. At present, it is widely planted in China, New Zealand, Italy, Iran, and so on [2,3]. Botryosphaeria dothidea, frequently isolated from rotted fruit, is one of the major causing agents of post-harvest rot of kiwifruit, an important disease of A. deliciosa [2]. B. dothidea is a fungal pathogen capable of infecting a wide range of woody plant species, and causes different disease phenotypes including fruit rot, dieback, stem canker, and tree death [12]

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