Identification and Detection Odontoglossum ringspot virus on Native Orchids Collection of Nurserys in Java, Indonesia

Abstract

Nature orchid are one of original floral in Indonesia. Virus infection is one of the limiting factor in the cultivation of orchid. Infection Odontoglossum ringspot virus (ORSV) was reported infets native orchids collection in Indonesia. The purpose of this study was to identification and ORSV that infects native orchid nurserys collection in Java, Indonesia. Symptomatic samples were collected from 5 nurserys collections, i.e. Rumah Bunga Rizal (Bandung), Bali Tanaman Hias (Cianjur), Borobudur Orchids Center (Magelang), Kebun Anggrek Bungarinte (Yogyakarta), and Titi Orchids (Yogyakarta). Detection and identification was conducted by serological test using ORSV specific antisera, RT-PCR and DNA sequencing. The serological test using ORSV antisera showed that 3 of 11 sampels reacted positively against ORSV antiserum i.e Phalaenopsis amabilis (Cianjur.1, Cianjur.2, and Magelang). RT-PCR of the 3 samples using specific primer of ORSV coat protein (CP) gene amplified a DNA with size ± 474 bp. Homology analysis of those 3 Indonesian isolates showed highest index similiarity (IS) was 99.8% with corresponding sequences from 10 other ORSV isolates. Phylogenetic analysis showed that ORSV Cianjur.1 and Cianjur.2 isolates clustered in separated group far from ORSV isolates in other countries.