Identification and comparative expression profiles of chemosensory genes in major chemoreception organs of a notorious pests, Laodelphax striatellus

Abstract

The small brown planthopper, Laodelphax striatellus (Stål) (SBPH), is a notorious rice pest in East Asia and damages the host by feeding on the phloem and transmitting virus particles. Although SBPH relies on chemosensory perception for seeking the host, courtship, selecting oviposition sites and spreading virus particles, a systematic study of chemosensory genes in SBPH is lacking. In this study, we identified multi-gene chemosensory families from the transcriptome of SBPH olfactory organs and analyzed their expression patterns in male and female tissues. Among the chemosensory genes, 14 odorant-binding proteins (OBPs), 12 chemosensory proteins (CSPs), 7 sensory neuron membrane proteins (SNMPs) and 95 odorant receptors (ORs) were identified and annotated in SBPH olfactory organs. Based on expression profile and phylogenetic analysis, LstrOBP1, 2, 5, 6, 7, 10, LstrSNMP1, and most LstrORs showed an antennae-enriched expression pattern, which suggests an olfactory role for these genes. Relative expression of LstrOBPs was validated by quantitative real-time PCR. Our findings provide the genetic information for disrupting the feeding behavior of SBPH, which is essential for developing eco-friendly pest management technologies.