Abstract
Juvenile hormone (JH) plays an important role in regulating growth, development, and reproduction of insects. Three key enzymes, namely, JH esterase (JHE), JH epoxide hydrolase (JHEH), and JH diol kinase (JHDK), are involved in JH degradation. In this study, we identified the full-length cDNAs of the 3 genes BdJHEH2, BdJHEH3, and BdJHDK encoding JHEH and JHDK from the oriental fruit fly, Bactrocera dorsalis (Hendel) (Diptera: Tephritidae). We used quantitative real-time polymerase chain reaction to investigate mRNA expression profiles of these 3 genes in various development stages and tissues, and in response to both 20-hydroxyecdysone (20E) and starvation. Both BdJHEH2 and BdJHDK were highly expressed during the larval–pupal transition, whereas BdJHEH3 was mainly expressed in the early 3rd instars. All 3 genes were highly expressed in 7-d-old and 10-d-old adults, but exhibited sex-specific expression patterns. BdJHEH2 was highly expressed in fat body, whereas BdJHEH3 and BdJHDK were most abundant in Malpighian tubules. In response to 20E exposure, the 3 genes were significantly up-regulated at various time points compared with the control. However, the transcript levels of BdJHDK decreased significantly during the initial exposure to 20E. After starvation treatment, expression of BdJHEH2 and BdJHEH3 significantly decreased, whereas BdJHDK was up-regulated. No significant change was observed after feeding resumption. These 3 genes have distinct roles in regulating growth and development of B. dorsalis. ResumenLa hormona juvenil (JH) juega un papel importante en la regulacion de crecimiento, el desarrollo y la reproduccion de los insectos. Tres enzimas clave, JH esterasa (JHE), JH epoxido hidrolasa (JHEH), y JH diol quinasa (JHDK), estan implicadas en la degradacion de JH. En este estudio, hemos identificado los ADNc de longitud completa de los 3 genes BdJHEH2, BdJHEH3 y BdJHDK que codifican JHEH y JHDK de la mosca oriental de la fruta, Bactrocera dorsalis (Hendel) (Diptera: Tephritidae). Se utilizo PCR cuantitativa en tiempo real para investigar los perfiles de expresion de mRNA de estos 3 genes en diferentes estadios de desarrollo y tejidos, y en respuesta a tanto 20-hidroxiecdisona (20E) y la inanicion. Tanto BdJHEH2 y BdJHDK fueron altamente expresado durante la transicion larval-pupal, mientras que BdJHEH3 se expreso principalmente en el 3er estadio temprano. Todos los 3 genes fueron altamente expresado en los adultos de 7 dias de edad y de 10 dias de edad, pero exhiben patrones de expresion especificos al sexo. BdJHEH2 fue altamente expresado en la grasa corporal, mientras que BdJHEH3 y BdJHDK fueron mas abundantes en los tubulos de Malpighi. En respuesta a la exposicion 20E, los 3 genes fueron significativamente hasta reguladas en varios puntos de tiempo en comparacion con el control. Sin embargo, los niveles de transcripcion de BdJHDK disminuyeron significativamente durante la exposicion inicial a 20E. Despues del tratamiento de inanicion, la expresion de BdJHEH2 y BdJHEH3 disminuyo significativamente, mientras que BdJHDK su regulacion aumento. No se observo ningun cambio significativo despues de la reanudacion de la alimentacion. Estos 3 genes tienen funciones distintas en la regulacion del crecimiento y el desarrollo de B. dorsalis. View this article in BioOne
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