Abstract
A new DNA repair gene from fission yeast Schizosaccharomyces pombe rlp1 + ( RecA- like protein) has been identified. Rlp1 shows homology to RecA-like proteins, and is the third S. pombe Rad51 paralog besides Rhp55 and Rhp57. The new gene encodes a 363 aa protein with predicted M r of 41,700 and has NTP-binding motif. The rlp1Δ mutant is sensitive to methyl methanesulfonate (MMS), ionizing radiation (IR), and camptothecin (CPT), although to a lesser extent than the deletion mutants of rhp55 + and rhp51 + genes. In contrast to other recombinational repair mutants, the rlp1Δ mutant does not exhibit sensitivity to UV light and mitomycin C (MMC). Mitotic recombination is moderately reduced in rlp1 mutant. Epistatic analysis of MMS and IR-sensitivity of rlp1Δ mutant indicates that rlp1 + acts in the recombinational pathway of double-strand break (DSB) repair together with rhp51 +, rhp55 +, and rad22 + genes. Yeast two-hybrid analysis suggests that Rlp1 may interact with Rhp57 protein. We propose that Rlp1 have an accessory role in repair of a subset of DNA damage induced by MMS and IR, and is required for the full extent of DNA recombination and cell survival under condition of a replication fork collapse.
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