Abstract
A bacterium, PG-3-2, capable of butane-utilization as a sole carbon source was isolated from Puguang oilfield in Sichuan Province, China and identified as Arthrobacter sp. by 16S rRNA gene sequence and morphology characteristics. Butane-saturated medium was defined as optimal for the growth of PG-3-2. Proliferation of PG-3-2 was enhanced at low butanol concentrations (≤50 mM) and repressed at high concentrations (≥100 mM). Growth of strain PG-3-2 was supported by alkanes from C2 to C10 (except pentane) and various carbon substrates including primary alcohols, secondary alcohols, carboxylic acids, aldehydes, ketones, but not methane or its oxidation products. The rate of butane degradation by PG-3-2 was relatively high during the lag phase and prophase of the exponential phase. A bmoX gene, which encodes the alpha hydroxylase subunit of butane monooxygenase, was amplified from the genome of this bacterium. Sequence analysis revealed a high level of homology with alkane monooxygenase, thus indicating the existence of a novel bmoX gene involved in the butane degradation pathway in this Arthrobacter strain.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have