Abstract
Squamosa promoter-binding protein-like (SPL) is a major family of plant-specific transcription factor, which is involved in multiple biological processes, such as plant growth and development, hormone response, light response and stress response. Therefore, it has been profoundly significant to systematically analyze the SPL Transcription Factors family in Brassica oleracea. In this study, a total of 33 BoSPLs were identified in the B. oleracea genome, and they were further divided into six subgroups based on the phylogenetic tree constructed from the SPL proteins of B. oleracea, B. rapa and Arabidopsis thaliana. The expression profile of BoSPLs in different organs/tissues showed that a large number of BoSPLs were expressed in the callus, root, stem, leaf, bud, flower and silique. In addition, the expression levels of two BoSPLs (BoSPL9b and BoSPL10b) were up-regulated in chilling tolerance cabbage ‘CT-923’ at 6 h after chilling stress when compared with normal treatment (mock), while two BoSPLs (BoSPL9b and BoSPL15a) in chilling sensitive cabbage ‘CS-D9’, five BoSPLs (BoSPL1, -9a, -9b, -10b, -11b) in ‘CT-923’ and two BoSPLs (BoSPL9b and BoSPL16a) in ‘CS-D9’ were up-regulated after 24 h chilling stress, indicated that these genes may play an important role in the chilling-tolerance of cabbage. We analyzed the characteristics of BoSPLs and provided the basis for further functional research.
Highlights
Squamosa promoter-binding protein-like (SPL) is a plant-specific transcription factor family, which plays an important role in plant growth and development [1], plant architecture [2], primary and secondary metabolism [3], signaling [4] and biotic stresses [5].SPL genes were first discovered in Antirrhinum majus [6], subsequently, several SPL families were identified in other species, such as Arabidopsis thaliana [7], rice [8,9], Chinese cabbage [10] and tomato [11]
The open reading frames (ORF) of BoSPLs ranged from 408 bp (BoSPL3c) to 4833 bp (BoSPL10c), and the corresponding proteins varied from 135 (BoSPL3c) to 1610 (BoSPL10c) amino acids, the predicted molecular weights ranged from 15.77 (BoSPL3c) to 178.85 (BoSPL10c) kDa
The results of the subcellular localization prediction showed that all the BoSPL proteins were in the nucleus, except for BoSPL19, which was in cytoplasm, and in secretory when Plant-mPLoc and BaCelLo were used, respectively (Table 1, Supplementary Table S3)
Summary
Squamosa promoter-binding protein-like (SPL) is a plant-specific transcription factor family, which plays an important role in plant growth and development [1], plant architecture [2], primary and secondary metabolism [3], signaling [4] and biotic stresses [5].SPL genes were first discovered in Antirrhinum majus [6], subsequently, several SPL families were identified in other species, such as Arabidopsis thaliana [7], rice [8,9], Chinese cabbage [10] and tomato [11]. Squamosa promoter-binding protein-like (SPL) is a plant-specific transcription factor family, which plays an important role in plant growth and development [1], plant architecture [2], primary and secondary metabolism [3], signaling [4] and biotic stresses [5]. The SPL protein contains a highly conserved DNA binding region, the SBP domain. The SBP domain is a typical zinc finger structure consisting of eight His or Cys residues, the C-terminus is Cys-Cys-His-Cys, the N-terminus is. Cys-Cys-Cys-His or Cys-Cys-Cys-Cys [12]. These amino acid residues can bind to a single zinc ion, when zinc ion is absent, the SBP domain does not have the ability to bind to DNA. The SPL genes mainly regulate the expression of downstream genes by binding to
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
