Identification and Characterization of Pseudomonas aeruginosa 16S rRNA Gene Isolated from Contaminated Soil With Oil Residues

Abstract

Pseudomonas aeruginosa was isolated from fifty soil samples were collected from different sites of contaminated soil with oil residues in Ministry of science& technology. Morphological, biochemical tests and 16S rRNA genes sequencing was performed for bacterial isolates identification. Thirty (30) isolates of P. aeruginosa bacteria were confirmed according to morphological, biochemical tests. For molecular analysis, all isolates that tested positive for P. aeruginosa underwent amplification of the 16S rRNA gene using previously described primers that amplified a particular DNA fragment of 956 bp. PCR product was delivered to Macrogen Corporation - Korea for Sanger sequencing utilizing an automated DNA sequencer called the ABI3730XL. The results were emailed to us, and we used geneious software to analyze them. The investigation of phylogenetic relationships between different strains of Pseudomonas aeruginosa has frequently been conducted on the sequencing of the 16S rRNA gene region, which is a viable technique for species identification. To determine the degree of genetic similarity between the organisms, a distance tree was built. Consequently, gene sequencing of the 16S rRNA region was an appropriate method for isolating isolates at the molecular level. The a novel local strain of Ps. aeruginosa which isolated from contaminated soil with oil residues samples (MSZ. IRQ20) and we will registration of isolate in GenBank under accession number of MT832126.1. Distance Tree Using Blast Tool in the Geneious software.