Identification and Characterization of Potential Phenol Degrading Bacterial strains Isolated from Municipal Sewage, Bilaspur, Chhattisgarh

Rashmi Parihar,Sumit Kumar Dubey

doi:10.3126/ijasbt.v4i3.15386

Abstract

Phenol and its derivatives are consistently causing harmful effects to an aquatic ecosystem. The present study focused on the isolation and characterization of potential phenol degrading bacterial strains and subsequently optimization of media ingredients for efficient phenol degradation by potential bacterial strains. Bacterial strains were isolated from municipal sewage, Bilaspur (21'47 and 23'8 N 81'14 and 83'15 E). After optimization phenol degradation rate was increased by 1.84 fold for PDB 5 (from 40.37% to 74.67%) and 1.39 fold for PDB 11 (from 58.62% to 81.51%) at 500mg/l initial phenol concentration. PDB 5 and PDB 11 were identified as Streptococcus sp. PDB 5 and Pseudomonas sp. PDB 11 respectively as potential phenol degrading bacterial strains. These strains can further be used in microbially assisted phenol degradation to remove phenol derivatives present in industrial wastewater.Int J Appl Sci Biotechnol, Vol 4(3): 288-293

Highlights

Effluents of Petrochemical, coke oven, dye industries, plastic and fiberglass manufacturing unit, pulp and paper production, herbicide manufacturing and oil refineries have been reported for higher amount of phenolic compounds (Paisio et al, 2013, Mohite et al, 2010, El-Ashtoukhy et al, 2013, Veeresh et al, 2005, Jadhav and Vanjara, 2004)
Initial phenol concentration was subtracted by residual phenol concentration for the assessment of phenol degrading efficiency of microbial strains
PDB 11 were identified as potential phenol degrading bacterial strains

Summary

Introduction

Effluents of Petrochemical, coke oven, dye industries, plastic and fiberglass manufacturing unit, pulp and paper production, herbicide manufacturing and oil refineries have been reported for higher amount of phenolic compounds (Paisio et al, 2013, Mohite et al, 2010, El-Ashtoukhy et al, 2013, Veeresh et al, 2005, Jadhav and Vanjara, 2004). Further phenolic compounds cause harm to aquatic living organisms (e.g. fish) and related organisms which depend on them as a food source (e.g. Eagle and humans). WHO quoted that short-term exposure of excess phenol inside human body causes liver and kidney damage, harmful effects on respiratory system and growth retardation

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