Identification and Characterization of Nuclear Matrix Protein(s) and assessment of its usefullness for early diagnosis of bladder cancer

Abstract

Background: the highest incidence rates of bladder cancer are generally found in industrially developed countries, particularly North America and Western Europe, and areas associated with endemic schistosomiasis, including parts of Africa and the Middle East . The appropriate treatment of patients with bladder cancer mandates early detection and regular follow up for recurrences . Currently, cystoscopy is the standard method for diagnosing and monitoring bladder cancer recurrence, but it is an invasive and relatively costly technique, and may sometimes be inconclusive, particularly in cases of cystitis . Patients and methods: western blot and specific immunoglobulin-G antibody were used to identify the urinary NMP marker. Urine samples from 123 patients with bladder cancer and 50 controls were evaluated using the developed SDS-PAGE, Western blot and ELISA. Results: the NMP marker was identified in the urine of patients with bladder cancer at 52 kDa (NMP- 52) by SDS-PAGE, Western blot and ELISA. In addition, the NMP-52 tumor marker was not detected in the urine of patients . Conclusion: detecting the urinary NMP-52 marker using SDS-PAGE, Western blot and ELISA, would be helpful in the rapid diagnosis of bladder cancer.