Abstract

Lipopolysaccharide induced TNFα factor (LITAF) is an important transcription factor responsible for regulation of tumor necrosis factor α. In this study, a novel litaf gene (designated as Malitaf) was identified and characterized from blunt snout bream, Megalobrama amblycephala. The full-length cDNA of Malitaf was of 956 bp, encoding a polypeptide of 161 amino acids with high similarity to other known LITAFs. A phylogenetic tree also showed that Malitaf significantly clustered with those of other teleost, indicating that Malitaf was a new member of fish LITAF family. The putative maLITAF protein possessed a highly conserved LITAF domain with two CXXC motifs. The mRNA transcripts of Malitaf were detected in all examined tissues of healthy M. amblycephala, including kidney, head kidney, muscle, liver, spleen, gill, and heart, and with the highest expression in immune organs: spleen and head kidney. The expression level of Malitaf in spleen was rapidly up-regulated and peaked (1.29-fold, p < 0.05) at 2 h after lipopolysaccharide (LPS) stimulation. Followed the stimulation of Malitaf, Matnfα transcriptional level was also transiently induced to a high level (51.74-fold, p < 0.001) at 4 h after LPS stimulation. Taken together, we have identified a putative fish LITAF ortholog, which was a constitutive and inducible immune response gene involved in M. amblycephala innate immunity during the course of a pathogenic infection.

Highlights

  • Blunt snout bream (Megalobrama amblycephala) is one of the major economically important species in freshwater polyculture fish aquaculture in China [1]

  • The complete Malitaf cDNA sequence was 956 bp, which was composed of an 88-bp length 5 -untranslated region (5 -UTR), a 486-bp open reading frame encoding a protein comprising 161 amino acids, and a 358-bp 3 -UTR followed by a poly (A) tail (Figure 1)

  • We identified and characterized a new member of LITAF family, Malitaf, in M. amblycephala

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Summary

Introduction

Blunt snout bream (Megalobrama amblycephala) is one of the major economically important species in freshwater polyculture fish aquaculture in China [1]. It has been widely cultured because of its herbivorous habit, faster growth rate, and delicate flesh quality, as well as increasing demand in China during the last few decades [2,3]. The infectious disease outbreak with quick spreading has led to serious economic losses in M. amblycephala culture industry. Innate immunity plays crucial roles in defense against bacterial infections in fish [6]. Finding more molecular components involved in M. amblycephala’s innate immunity, will facilitate our understandings in the largely unveiled complex immunity in fish

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