Abstract
Tef [Eragrostis tef (Zucc.) Trotter] is becoming popular for its healthy diet and resilience to changes in climate. TFs are genes regulating in the transcription through binding to specific sequences or by protein-protein interaction. Dofs are plant specific TFs and involved in different plant specific processes. Little is known about their features in tef and comparative phylogenetic relationship of Dof proteins with other plants. In silico identification is a popular method to identify functional genes in almost every plant. Thirty three (33) Dof TFs have been identified through BLAST search in the available databases. Multiple sequence alignment and phylogenetic tree were constructed using MEGA X software for Sorghum bicolor, Setaria italica, Oryza sativa, Arabidopsis thaliana and Populus trichocarpa Dof proteins depicting evolutionary relationship in ten major clusters. The promoter analysis revealed the existence of light related/responsive and cis- acting regulatory elements in stress condition implying their role in photosynthesis and response in biotic and abiotic stresses. The study unravels information on the structure and function of Dof genes in Eragrostis tef in particular and related cereals in general.
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