Abstract
Berberis vulgaris L is a shrub of family Berberidaceae produces in Asia and Europe A broad range of medicinally and nutritionally important phytochemical components have been isolated from B vulgaris which are of medicinal value Berberine is the main alkaloid isolated from B vulgaris Chromatography was performed on an Acquity UPLC system Waters equipped with a HSS T column using mixture of water containing formic acid and acetonitrile v v as mobile phase The mass spectrometric detection was performed by selected ion monitoring mode via electrospray ionization in the positive ionisation mode The results indicate that the approximate berberine content in the B vulgaris root extract as determined by the proposed method was mg ml The fully validated HR LC MS method can be successfully applied for the determination of berberine in B vulgaris which can be used as a potent therapeutic agent for the treat of hypopigmentary disorders
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