Abstract
This study aimed to explore the potency of Gonggong sea snail's (GSS) extract as an antimicrobial peptide (AMP) source. The results showed that the GSS meat extracts exhibited potential antimicrobial activity against Staphylococcus aureus and Escherichia coli. A peptide band with a molecular weight < 5kDa was obtained for the characterization of AMP candidates after separating the selected extract using SDS-PAGE, and the sequences were acquired by LC-ESI-MS analysis. The results of the bioinformatics analysis showed that the AMP candidate had a molecular weight of 1.4kDa, which consisted of 12 amino acid residues (RHPDYSVALLLR), with an α-helix structure, isoelectric point pH (pI) of 9.53, net charge + 1, a total hydrophobic ratio at 49.9%, protein-binding potential (Boman index) of 2.17kcal/mol, and hydrophobicity of + 13.67kcal/mol. Furthermore, MIC and MBC values of the extract and the < 10kDa fraction on both bacteria ranged from 0.50-1.03mg/ml. The GSS meat extract could reach the intracellular site of E. coli, while in S. aureus, it was localized in the cell membrane. These results can be baseline information for developing AMPs in natural bio-preservative exploration as food additives and pharmaceuticals.
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