Abstract
This study demonstrated that the vitamin D3 decomposition product VDP1 exerts an antibacterial action against Helicobacter pylori but not against other bacteria. Treatment with VDP1 induced a collapse of cell membrane structures of H. pylori and ultimately lysed the bacterial cells. A unique dimyristoyl phosphatidylethanolamine in the membrane lipid compositions contributed to the interaction of VDP1 with H. pylori cells. In separate experiments, VDP1 had no influence on the viability of the human cancer cell lines MKN45 and T47D and lacked any vitamin D3-like hormonal action against the latter. In both 1H and 13C NMR analyses, the spectra patterns of VDP1 corresponded with those of Grundmann's ketone. These results suggest that VDP1 (or Grundmann's ketone-type indene compound) may become a fundamental structure for the development of new antibacterial substances with selective bactericidal action against H. pylori.
Highlights
This study demonstrated that the vitamin D3 decomposition product VDP1 exerts an antibacterial action against Helicobacter pylori but not against other bacteria
We have found that dimyristoyl PE (DMPE) is one of the most prevalent PE molecular species in H. pylori cell membrane lipid compositions
We examined whether VDP1 and VDP2 derived from a steroidal compound have a higher binding affinity to PE molecular species carrying two myristic acid molecules (DMPE) than to PE molecular species carrying two palmitic acid molecules (DPPE)
Summary
This study demonstrated that the vitamin D3 decomposition product VDP1 exerts an antibacterial action against Helicobacter pylori but not against other bacteria. Recent studies by our group have revealed that phosphatidylethanolamine (PE) in H. pylori lipid compositions carries a myristic acid molecule, has a high binding affinity to non-esterified steroidal compounds such as free cholesterol, pregnenolone, and progesterone at the carbon-3 position in its frames, and plays an important role in the interaction between the bacterial cells and these steroidal compounds[6,7]. Progesterone and its analogues induce the cell lysis of H. pylori by binding to the myristoyl PE molecular species of the bacterial cells[7,8] These investigations by our group suggest that chemical compounds with high binding affinity to the H. pylori PE may confer selective antibacterial action against this bacterium. We analyzed the interaction of vitamin D3 species with H. pylori cells
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