Abstract
Cells of the human erythroleukemia cell line K562 constitutively secrete a factor that inhibits human T lymphocyte proliferation induced via CD3 Ti . The factor, termed K-TIF (K562-derived T cell inhibitory factor) is produced in either the presence or absence of fetal calf serum in cultures of K562 cells and can be precipitated by 70% NH 4SO 4. Gel flitration chromatography on Superose 12 resin by FPLC showed that the inhibitory factor has a molecular weight of approximately 30–35 kDa. A protein of this size, metabolically labeled with [ 35S]methionine, specifically bound human peripheral blood mononuclear cells. Chromatofocusing with Mono P by FPLC (pH gradient 7.2–5) indicates that the inhibitory factor has an isoelectric point of 6.0–6.4.
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