Abstract
By conducting a systemic screening from Chinese traditional Tofu, a bacteriocin producer with broad antimicrobial spectrum was found. The producer was identified by 16S rDNA sequencing, and it was identified as <i>Leuconostoc mesenteroides</i>. The producer possessed typical bacteriocin traits, such as be heat-stable and proteinase-labile. Its inhibition spectrum contained many distantly related genera of Firmicutes, comprising most lactic acid bacteria (LAB) as well as problematic species of <i>Bacillus, Listeria</i> and <i>Staphylococcus</i>. In addition, the bacteriocin producer showed significantly inhibition effects on controlling <i>Listeria monocytogenes</i> in milk. Taken together, the results indicate that this is a novel and potent bacteriocin from <i>Leu.mesenteroides</i> and that its very broad inhibition spectrum can be use in food preservation as well as in infection treatments caused by Gram-positive pathogens.
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